Electronic hookah vaping reduces plasma concentrations of nitrate/nitrite and increases systemic oxidative stress

Abstract Background Electronic (e-) hookah–a new category of vaping devices–has increased in use among young adults recent years. E-hookahs utilize the placement e-bowls on traditional water pipes, allowing flavored aerosol to pass through a water-filled base before being inhaled. Though advertised as safe tobacco alternative, e-hookahs deliver nicotine by creating an fine particles and other free radicals that may increase cardiovascular disease risk. While acute e-hookah impairs endothelial function, underlying mechanism is not completely understood. Purpose To determine mechanistic involvement nitric oxide (NO) oxidative stress induced dysfunction. Methods Healthy adult chronic hookah smokers (n=7; 26±1 years age, 24.4±0.8 kg-m2; mean ±SE) were randomized vape two 30-minute sessions sham vaping, separated 7-day washout period. Flow-mediated dilation (FMD) brachial artery was performed with ultrasound. Circulating plasma nitrite/nitrate (NOx), index concentration, lipid peroxidation marker 8-isoprostane concentrations, well smoking exposure biomarkers (plasma exhaled carbon monoxide) measured after sessions. Results As compared unchanged parameters (p=ns), reduction FMD −27±3% (pre- vs. post-vaping, p<0.001), suggesting impairment function. These vascular changes accompanied significant reductions circulating levels NOx increases 8-iso prostaglandin F2a (−41±11% +28±11%, respectively; p<0.001). Plasma concentrations 4-fold more e-hookah, whereas CO did change (p=ns). Conclusions Our data demonstrate transiently systemic decreases levels, latter implies reduced NO bioavailability line observed expressed reductions. Thus, contrast unsubstantiated beliefs benign, overtly healthy adults, causes functional impairment, likely mediated stress. Funding Acknowledgement Type funding sources: Public Institution(s). Main source(s): University California Tobacco-Related Disease Research Program,